However, the flavoring is often a combination of aromatic and flavor compounds discovered through the study of plants in laboratories that have little to do with cherry trees.
But now a team of Purdue University researchers has discovered the molecular recipe for one of the most coveted compounds by the flavor industry: benzaldehyde. The compound is key to some of the most popular flavors including cherry, almond and raspberry. It is second only to vanillin in terms of economic value to the food industry.
“Benzaldehyde is what gives that pleasant almond-like scent and is part of the aroma of many fruits,” said Natalia Dudareva, Distinguished Professor of Biochemistry in Purdue’s College of Agriculture, who led the team.
“That scent attracts pollinators and, in addition to those fruits, it is found in other plants, including petunias.”
Biochemists figure out molecular ‘recipes’, or biosynthetic pathways, to make compounds for use as different flavors for the products we consume. However, when steps in the molecular recipe can’t be found, chemicals are often substituted for commercial production, she explained.
“When chemical reactions are added in to fill the gaps, it can be a problem,” Dudareva, who is also director of Purdue’s Center for Plant Biology, said.
“It is much better and safer to use an entirely natural pathway to a flavor compound, but it is difficult to discover all of the steps. Benzaldehyde has an especially puzzling biosynthetic pathway, and it wasn’t completely revealed until now.”
Dudareva and her team studied the scent of petunia flowers to discover the molecular recipe for benzaldehyde. The work is detailed in a paper in Nature Communications, and a patent on the process is pending.
“The goal is to have the natural process found in plants, and this discovery makes that possible for a very important flavor compound,” Dudareva said.
“The alternative to synthesis is truly extracting a compound from plants, but only 1.5% of the benzaldehyde in worldwide production is obtained this way.”
The biosynthetic pathway will likely be genetically transferred to yeast or other microbes to incorporate it into the fermentation process widely used in food and beverage production, she said.
The team found synthesis of benzaldehyde in petunia petals involves an enzyme consisting of two subunits that must combine in equal amounts to activate, said Xing-Qi Huang, an author of the paper and postdoctoral researcher in Dudareva’s lab.
“The gene directly responsible and enzyme needed for benzaldehyde synthesis were a mystery,” he said.
“We tried newer techniques, but it took a classical approach to reveal it.”
This was because of the enzyme’s uncommon need for two subunits to function, or heterodimeric structure, he said.
Because of this, earlier analysis methods looking for a single component appeared to fail.
“We estimate the size of the protein we are hunting in addition to other things we have learned about the pathway,” he said.
“We weren’t finding a good indication of a single protein within that estimate. However, we noticed the presence of two components of half the size of our estimate, and we thought maybe there are two subunits.”
Further testing confirmed their idea and revealed the genes involved. Their work found the protein subunits form what is called a Rossmann fold, named after the late Purdue professor and structural biologist Michael Rossmann.
“Purdue is all over the petunia,” Dudareva said.
“This discovery is the most recent. We now have mapped out almost all of the genes and pathways responsible for petunia scent compounds. To also see within it proteins that embody a structure discovered by and named after a fellow faculty member adds a special connection. It is beautiful.”
In addition to Dudareva and Huang, Renqiuguo Li and Jianxin Fu participated in the research and co-authored the paper.
The National Science Foundation (IOS-1655438) and the USDA National Institute of Food and Agriculture (Hatch Project number 17784) funded the research.