After eggs, Australian research device cracks milk protein on route to disease therapies
The vortex fluidic device (VFD) has previously been used in experiments to successfully 'un-boil' egg protein and break the molecular bonds of one of the world's hardest material, carbon nanotubes.
In the latest application, published in Molecules, College of Science and Engineering researchers combined the capabilities of the VFD with a new form of biosensor, TPE-MI, which is an aggregation-induced emission luminogen (AIEgen).
"In the human body, protein folding is a regular process which in some cases may involve misfolding and aggregation such as in gene mutation, which can upset the balance," said Professor Youhong Tang, whose research focuses on expanding AIEgen technologies.
"One is example is the build-up of amyloid proteins, which is associated with diseases such as Alzheimer's, Parkinson's and Huntington's. Finding ways to monitor these protein levels - and even reversing high levels of these cellular aggregations - could lead to future therapies."
SA Scientist of the Year Professor Colin Raston, who designed the VFD, said the combination of both technologies produced some promising results in fields of medical discovery.
"In this latest study, we showed how vital proteins can be manipulated - unfolded and refolded - using β-lactoglobulin, which is a relatively simple, low molecular weight protein.
"Combining VFD and AIE technologies provides a fully capable and robust method for controlling and monitoring the progress of protein denaturation and renaturation."
The research team will now use the technology combination on other proteins, focusing on those highly related to Alzheimer's disease, Parkinson's disease, and Huntington's disease.
In Situ Monitored Vortex Fluidic-Mediated Protein Refolding/Unfolding Using an Aggregation-Induced Emission Bioprobe (2021)
Qi Hu, H Hu, X Zhang, K Fan,Y Hong, CL Raston and Y Tang
Molecules Vol 26, Issue 14
https://www.mdpi.com/1420-3049/26/14/4273/htm#
